An immunotyping of 27 strains of Chlamydia trachomatis isolated from patients with nongonococcal urethritis or pelvic inflammatory diseases in Korea was achieved in dot-enzyme linked immunosorbent assay (dot-ELISA) with monoclonal antibodies.
Monoclonal
antibodies were produced with standard techniques by immunization of Balb/c mice and fusion with SP 2/0 myeloma cell. Seven type-specific (D, E, F, H, I, J, L2), 2 subspecies-specific (B-complex, C-complex) and speciesspecific (HMC 1-1)
monoclonal
antibodies were used for immunotyping. Immunotyping of 12 control strains and 27 clinical strains isolated in Korea was studied by using dot-ELISA.
Species-specific (HMC 1-1) monoclonal antibody reacted with all control strains and 27 isolated. Subspecies-specific (B-complex) monoclonal antibody reacted with B/HAR-36, Ba/Aphach-2, D/UW-3Cx, E/Bour, LGV type I/440, LGV type II/CDC control
strains
and 19 isolates. Type-specific monoclonal antibody of D was reacted with D/UW-3/Cx control strain and 10 isolates. E type-specific monoclonal antibody reacted with E/Bour control strain and 6 isolates. F type-specific monoclonal antibody reacted
with 5
isolates. Three isolates which reacted with subspecies-specific monoclonal antibody didn't react with any type-specific monoclonal antibodies. Subspecies-specific (C-complex) monoclonal antibody reacted with A/HAR-13, C/CDC, H/UW-43/Cx,
J/UW-36/Cx
control strains and 2 clinical isolates, but the isolates did not react with any type-specific monoclonal antibodies. One of 27 isolates reacted with any type-specific monoclonal antibodies. One of 27 isolates reacted with species-specific (HMC
1-1)
monoclonal antibody didn't react with any other subspecies-and typespecific monoclonal antibodies.
In conclusion, the major immunotypes of C. trachomatis from urogenital system in Korea were D, E and F, and dot-ELISA with monoclonal antibody may contribute for immunotyping as a simple and specific technique.
|